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r045a mouse il  (TaKaRa)


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    TaKaRa r045a mouse il
    R045a Mouse Il, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 5124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/r045a+mouse+il/PrimeSTAR+Max+DNA+Polymerase/pm41557504-214-67-92
    Average 99 stars, based on 5124 article reviews
    r045a mouse il - by Bioz Stars, 2026-09
    99/100 stars

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    Recombinant:

    Article Title: H3K27-specific demethylase orchestrates the phenotypic and functional plasticity of intestinal ILC3s.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Alexa Fluor TM 594 Donkey Anti-Rabbit IgG (H + L) Cell Signaling Cat# A21207; RRID: AB_2340621 Normal Rabbit IgG (2729) Cell Signaling Cat# 2729S; Anti-Rabbit IgG (H + L) Sigma-Aldrich Cat# SAB3700883; Bacterial and virus strains DH5α Chemically Competent Cell Tsingke Biotech Cat#TSC-C14 Citrobacter rodentium strain DBS100 ATCC Stock# 51459 Candida albicans strain SC5314. .. Guanghua Huang Lab, Fudan University Gifted from Prof. Guanghua Huang Chemicals, peptides, and recombinant proteins Fixable Viability Dye 450 eBioscience Cat#65-0863-14 Fixable Viability Stain 510 BD Cat#564406 FBS Gibco Cat#10270 PMA Sigma-Aldric Cat# P1585 DTT, Molecular Grade (Dry Powder) Promega Cat#V3151 Ionomycin Sigma-Aldric Cat# I3909 Polybrene Millpore Cat# TR-1003-G DNase I Sigma Cat# DN25 Collagenase VIII Sigma Aldrich Cat#C2139 Percoll Cytiva Cat#17-0891-01 PrimeSTAR MAX Premix TAKARA Cat# R045A Mouse IL-23 Recombinant Protein Peprotech Cat# 14-8231-63 IL-1β Peprotech Cat# SRP8033 Interleukin-7 Peprotech Cat# I4892 IL-6 Peprotech Cat# SRP3330 Murine SCF Peprotech Cat#250-03-50 RetroNectin Takara Cat#T100A/B Critical commercial assays BD Rhapsody TM WTA Amplification Kit BD Biosciences Cat# 633801 AMPure® XP magnetic beads Beckman Cat# A63880 Qubit TM dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q32851 BD Rhapsody TM Cartridge Reagent Kit BD Biosciences Cat# 633731 BD Rhapsody TM cDNA Kit BD Biosciences Cat# 633773 Deposited data scRNA-seq This paper GSE271288 CUT&Tag sequencing This paper GSE281331 Experimental models: cell lines HEK 293T ATCC Cat#ACS-4500 Plat E Shanghai Institutes for Biological Sciences Gifted from Prof. Xiaolong Liu Experimental models: organisms/strains Mouse:Kdm6a flox/flox (Utx) Jackson Laboratories Strain #:021926 RRID:IMSR_JAX:021926 Mouse:Kdm6b flox/flox (Jmjd3) Jackson Laboratories Strain #:029615 RRID:IMSR_JAX:029615 Mouse:Rorc-cre Jackson Laboratories Strain #: 022791 RRID:IMSR_JAX:022791 Mouse: NCG GemPharmatech (Nanjing, China) Strain #:T001475 Mouse:CD45.1 Jackson Laboratories Strain #: 002014 RRID:IMSR_JAX:002014 Deposited data RNAseq data This paper CRA033355 CUT&Tag This paper CRA033268 (Continued on next page) Cell Reports 45, 116862, January 27, 2026 17 .. The Jmjd3 f/f and Utx f/f mouse strain were purchased from the Jackson Laboratory (Bar Harbor, ME).

    Article Title: The transcription factor IRF4 regulates the homeostasis and function of intestinal ILC3s.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER IRF-4 Rabbit mAb (D9P5H) Cell Signaling Cat# 15106T; RRID: AB_2798709 Anti-BATF (EPR21911) Abcam Cat# ab221146; RRID: AB_3665801 Alexa Fluor TM 594 Donkey Anti-Rabbit IgG (H + L) Cell Signaling Cat# A21207; RRID: AB_2340621 Normal Rabbit IgG (2729) Cell Signaling Cat# 2729S; RRID:AB_1031062 Anti-Rabbit IgG (H + L) Sigma-Aldrich Cat# SAB3700883; RRID: AB_3697514 Bacterial and virus strains DH5α Chemically Competent Cell Tsingke Biotech Cat#TSC-C14 Citrobacter rodentium strain DBS100 ATCC Stock# 51459 Candida albicans strain SC5314. .. Guanghua Huang Lab, Fudan University Gifted from Prof. Guanghua Huang Chemicals, peptides, and recombinant proteins Fixable Viability Dye 450 eBioscience Cat#65-0863-14 Fixable Viability Stain 510 BD Cat#564406 FBS Gibco Cat#10270 PMA Sigma-Aldric Cat# P1585 DTT, Molecular Grade (Dry Powder) Promega Cat#V3151 Ionomycin Sigma-Aldric Cat# I3909 Polybrene Millpore Cat# TR-1003-G DNase I Sigma Cat# DN25 Collagenase VIII Sigma Aldrich Cat#C2139 Percoll Cytiva Cat#17-0891-01 PrimeSTAR MAX Premix TAKARA Cat# R045A Mouse IL-23 Recombinant Protein Peprotech Cat# 14-8231-63 IL-1β Peprotech Cat# SRP8033 Interleukin-7 Peprotech Cat# I4892 IL-6 Peprotech Cat# SRP3330 Murine SCF Peprotech Cat#250-03-50 RetroNectin Takara Cat#T100A/B Critical commercial assays BD Rhapsody TM WTA Amplification Kit BD Biosciences Cat# 633801 AMPure® XP magnetic beads Beckman Cat# A63880 Qubit TM dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q32851 BD Rhapsody TM Cartridge Reagent Kit BD Biosciences Cat# 633731 BD Rhapsody TM cDNA Kit BD Biosciences Cat# 633773 Deposited data scRNA-seq This paper GSE271288 CUT&Tag sequencing This paper GSE281331 Experimental models: Cell lines HEK 293T ATCC Cat#ACS-4500 Plat E Shanghai Institutes for Biological Sciences Gifted from Prof. Xiaolong Liu Experimental models: Organisms/strains Mouse:Irf4 flox/flox Jackson Laboratories Strain #:009380 RRID:IMSR_JAX:009380 Mouse:Rorc-cre Jackson Laboratories Strain #: 022791 RRID:IMSR_JAX:022791 Mouse: NCG GemPharmatech (Nanjing, China) Strain #:T001475 Mouse:CD45.1 Jackson Laboratories Strain #: 002014 RRID:IMSR_JAX:002014 Mouse:OT-II Jackson Laboratories Strain #:004194 RRID:IMSR_JAX:004194 (Continued on next page) e2 iScience 28, 112800, July 18, 2025 .. The Irf4 f/f mouse strain was bought from the Jackson Laboratory (Bar Harbor, ME).

    Staining:

    Article Title: H3K27-specific demethylase orchestrates the phenotypic and functional plasticity of intestinal ILC3s.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Alexa Fluor TM 594 Donkey Anti-Rabbit IgG (H + L) Cell Signaling Cat# A21207; RRID: AB_2340621 Normal Rabbit IgG (2729) Cell Signaling Cat# 2729S; Anti-Rabbit IgG (H + L) Sigma-Aldrich Cat# SAB3700883; Bacterial and virus strains DH5α Chemically Competent Cell Tsingke Biotech Cat#TSC-C14 Citrobacter rodentium strain DBS100 ATCC Stock# 51459 Candida albicans strain SC5314. .. Guanghua Huang Lab, Fudan University Gifted from Prof. Guanghua Huang Chemicals, peptides, and recombinant proteins Fixable Viability Dye 450 eBioscience Cat#65-0863-14 Fixable Viability Stain 510 BD Cat#564406 FBS Gibco Cat#10270 PMA Sigma-Aldric Cat# P1585 DTT, Molecular Grade (Dry Powder) Promega Cat#V3151 Ionomycin Sigma-Aldric Cat# I3909 Polybrene Millpore Cat# TR-1003-G DNase I Sigma Cat# DN25 Collagenase VIII Sigma Aldrich Cat#C2139 Percoll Cytiva Cat#17-0891-01 PrimeSTAR MAX Premix TAKARA Cat# R045A Mouse IL-23 Recombinant Protein Peprotech Cat# 14-8231-63 IL-1β Peprotech Cat# SRP8033 Interleukin-7 Peprotech Cat# I4892 IL-6 Peprotech Cat# SRP3330 Murine SCF Peprotech Cat#250-03-50 RetroNectin Takara Cat#T100A/B Critical commercial assays BD Rhapsody TM WTA Amplification Kit BD Biosciences Cat# 633801 AMPure® XP magnetic beads Beckman Cat# A63880 Qubit TM dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q32851 BD Rhapsody TM Cartridge Reagent Kit BD Biosciences Cat# 633731 BD Rhapsody TM cDNA Kit BD Biosciences Cat# 633773 Deposited data scRNA-seq This paper GSE271288 CUT&Tag sequencing This paper GSE281331 Experimental models: cell lines HEK 293T ATCC Cat#ACS-4500 Plat E Shanghai Institutes for Biological Sciences Gifted from Prof. Xiaolong Liu Experimental models: organisms/strains Mouse:Kdm6a flox/flox (Utx) Jackson Laboratories Strain #:021926 RRID:IMSR_JAX:021926 Mouse:Kdm6b flox/flox (Jmjd3) Jackson Laboratories Strain #:029615 RRID:IMSR_JAX:029615 Mouse:Rorc-cre Jackson Laboratories Strain #: 022791 RRID:IMSR_JAX:022791 Mouse: NCG GemPharmatech (Nanjing, China) Strain #:T001475 Mouse:CD45.1 Jackson Laboratories Strain #: 002014 RRID:IMSR_JAX:002014 Deposited data RNAseq data This paper CRA033355 CUT&Tag This paper CRA033268 (Continued on next page) Cell Reports 45, 116862, January 27, 2026 17 .. The Jmjd3 f/f and Utx f/f mouse strain were purchased from the Jackson Laboratory (Bar Harbor, ME).

    Article Title: The transcription factor IRF4 regulates the homeostasis and function of intestinal ILC3s.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER IRF-4 Rabbit mAb (D9P5H) Cell Signaling Cat# 15106T; RRID: AB_2798709 Anti-BATF (EPR21911) Abcam Cat# ab221146; RRID: AB_3665801 Alexa Fluor TM 594 Donkey Anti-Rabbit IgG (H + L) Cell Signaling Cat# A21207; RRID: AB_2340621 Normal Rabbit IgG (2729) Cell Signaling Cat# 2729S; RRID:AB_1031062 Anti-Rabbit IgG (H + L) Sigma-Aldrich Cat# SAB3700883; RRID: AB_3697514 Bacterial and virus strains DH5α Chemically Competent Cell Tsingke Biotech Cat#TSC-C14 Citrobacter rodentium strain DBS100 ATCC Stock# 51459 Candida albicans strain SC5314. .. Guanghua Huang Lab, Fudan University Gifted from Prof. Guanghua Huang Chemicals, peptides, and recombinant proteins Fixable Viability Dye 450 eBioscience Cat#65-0863-14 Fixable Viability Stain 510 BD Cat#564406 FBS Gibco Cat#10270 PMA Sigma-Aldric Cat# P1585 DTT, Molecular Grade (Dry Powder) Promega Cat#V3151 Ionomycin Sigma-Aldric Cat# I3909 Polybrene Millpore Cat# TR-1003-G DNase I Sigma Cat# DN25 Collagenase VIII Sigma Aldrich Cat#C2139 Percoll Cytiva Cat#17-0891-01 PrimeSTAR MAX Premix TAKARA Cat# R045A Mouse IL-23 Recombinant Protein Peprotech Cat# 14-8231-63 IL-1β Peprotech Cat# SRP8033 Interleukin-7 Peprotech Cat# I4892 IL-6 Peprotech Cat# SRP3330 Murine SCF Peprotech Cat#250-03-50 RetroNectin Takara Cat#T100A/B Critical commercial assays BD Rhapsody TM WTA Amplification Kit BD Biosciences Cat# 633801 AMPure® XP magnetic beads Beckman Cat# A63880 Qubit TM dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q32851 BD Rhapsody TM Cartridge Reagent Kit BD Biosciences Cat# 633731 BD Rhapsody TM cDNA Kit BD Biosciences Cat# 633773 Deposited data scRNA-seq This paper GSE271288 CUT&Tag sequencing This paper GSE281331 Experimental models: Cell lines HEK 293T ATCC Cat#ACS-4500 Plat E Shanghai Institutes for Biological Sciences Gifted from Prof. Xiaolong Liu Experimental models: Organisms/strains Mouse:Irf4 flox/flox Jackson Laboratories Strain #:009380 RRID:IMSR_JAX:009380 Mouse:Rorc-cre Jackson Laboratories Strain #: 022791 RRID:IMSR_JAX:022791 Mouse: NCG GemPharmatech (Nanjing, China) Strain #:T001475 Mouse:CD45.1 Jackson Laboratories Strain #: 002014 RRID:IMSR_JAX:002014 Mouse:OT-II Jackson Laboratories Strain #:004194 RRID:IMSR_JAX:004194 (Continued on next page) e2 iScience 28, 112800, July 18, 2025 .. The Irf4 f/f mouse strain was bought from the Jackson Laboratory (Bar Harbor, ME).

    Amplification:

    Article Title: H3K27-specific demethylase orchestrates the phenotypic and functional plasticity of intestinal ILC3s.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Alexa Fluor TM 594 Donkey Anti-Rabbit IgG (H + L) Cell Signaling Cat# A21207; RRID: AB_2340621 Normal Rabbit IgG (2729) Cell Signaling Cat# 2729S; Anti-Rabbit IgG (H + L) Sigma-Aldrich Cat# SAB3700883; Bacterial and virus strains DH5α Chemically Competent Cell Tsingke Biotech Cat#TSC-C14 Citrobacter rodentium strain DBS100 ATCC Stock# 51459 Candida albicans strain SC5314. .. Guanghua Huang Lab, Fudan University Gifted from Prof. Guanghua Huang Chemicals, peptides, and recombinant proteins Fixable Viability Dye 450 eBioscience Cat#65-0863-14 Fixable Viability Stain 510 BD Cat#564406 FBS Gibco Cat#10270 PMA Sigma-Aldric Cat# P1585 DTT, Molecular Grade (Dry Powder) Promega Cat#V3151 Ionomycin Sigma-Aldric Cat# I3909 Polybrene Millpore Cat# TR-1003-G DNase I Sigma Cat# DN25 Collagenase VIII Sigma Aldrich Cat#C2139 Percoll Cytiva Cat#17-0891-01 PrimeSTAR MAX Premix TAKARA Cat# R045A Mouse IL-23 Recombinant Protein Peprotech Cat# 14-8231-63 IL-1β Peprotech Cat# SRP8033 Interleukin-7 Peprotech Cat# I4892 IL-6 Peprotech Cat# SRP3330 Murine SCF Peprotech Cat#250-03-50 RetroNectin Takara Cat#T100A/B Critical commercial assays BD Rhapsody TM WTA Amplification Kit BD Biosciences Cat# 633801 AMPure® XP magnetic beads Beckman Cat# A63880 Qubit TM dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q32851 BD Rhapsody TM Cartridge Reagent Kit BD Biosciences Cat# 633731 BD Rhapsody TM cDNA Kit BD Biosciences Cat# 633773 Deposited data scRNA-seq This paper GSE271288 CUT&Tag sequencing This paper GSE281331 Experimental models: cell lines HEK 293T ATCC Cat#ACS-4500 Plat E Shanghai Institutes for Biological Sciences Gifted from Prof. Xiaolong Liu Experimental models: organisms/strains Mouse:Kdm6a flox/flox (Utx) Jackson Laboratories Strain #:021926 RRID:IMSR_JAX:021926 Mouse:Kdm6b flox/flox (Jmjd3) Jackson Laboratories Strain #:029615 RRID:IMSR_JAX:029615 Mouse:Rorc-cre Jackson Laboratories Strain #: 022791 RRID:IMSR_JAX:022791 Mouse: NCG GemPharmatech (Nanjing, China) Strain #:T001475 Mouse:CD45.1 Jackson Laboratories Strain #: 002014 RRID:IMSR_JAX:002014 Deposited data RNAseq data This paper CRA033355 CUT&Tag This paper CRA033268 (Continued on next page) Cell Reports 45, 116862, January 27, 2026 17 .. The Jmjd3 f/f and Utx f/f mouse strain were purchased from the Jackson Laboratory (Bar Harbor, ME).

    Article Title: The transcription factor IRF4 regulates the homeostasis and function of intestinal ILC3s.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER IRF-4 Rabbit mAb (D9P5H) Cell Signaling Cat# 15106T; RRID: AB_2798709 Anti-BATF (EPR21911) Abcam Cat# ab221146; RRID: AB_3665801 Alexa Fluor TM 594 Donkey Anti-Rabbit IgG (H + L) Cell Signaling Cat# A21207; RRID: AB_2340621 Normal Rabbit IgG (2729) Cell Signaling Cat# 2729S; RRID:AB_1031062 Anti-Rabbit IgG (H + L) Sigma-Aldrich Cat# SAB3700883; RRID: AB_3697514 Bacterial and virus strains DH5α Chemically Competent Cell Tsingke Biotech Cat#TSC-C14 Citrobacter rodentium strain DBS100 ATCC Stock# 51459 Candida albicans strain SC5314. .. Guanghua Huang Lab, Fudan University Gifted from Prof. Guanghua Huang Chemicals, peptides, and recombinant proteins Fixable Viability Dye 450 eBioscience Cat#65-0863-14 Fixable Viability Stain 510 BD Cat#564406 FBS Gibco Cat#10270 PMA Sigma-Aldric Cat# P1585 DTT, Molecular Grade (Dry Powder) Promega Cat#V3151 Ionomycin Sigma-Aldric Cat# I3909 Polybrene Millpore Cat# TR-1003-G DNase I Sigma Cat# DN25 Collagenase VIII Sigma Aldrich Cat#C2139 Percoll Cytiva Cat#17-0891-01 PrimeSTAR MAX Premix TAKARA Cat# R045A Mouse IL-23 Recombinant Protein Peprotech Cat# 14-8231-63 IL-1β Peprotech Cat# SRP8033 Interleukin-7 Peprotech Cat# I4892 IL-6 Peprotech Cat# SRP3330 Murine SCF Peprotech Cat#250-03-50 RetroNectin Takara Cat#T100A/B Critical commercial assays BD Rhapsody TM WTA Amplification Kit BD Biosciences Cat# 633801 AMPure® XP magnetic beads Beckman Cat# A63880 Qubit TM dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q32851 BD Rhapsody TM Cartridge Reagent Kit BD Biosciences Cat# 633731 BD Rhapsody TM cDNA Kit BD Biosciences Cat# 633773 Deposited data scRNA-seq This paper GSE271288 CUT&Tag sequencing This paper GSE281331 Experimental models: Cell lines HEK 293T ATCC Cat#ACS-4500 Plat E Shanghai Institutes for Biological Sciences Gifted from Prof. Xiaolong Liu Experimental models: Organisms/strains Mouse:Irf4 flox/flox Jackson Laboratories Strain #:009380 RRID:IMSR_JAX:009380 Mouse:Rorc-cre Jackson Laboratories Strain #: 022791 RRID:IMSR_JAX:022791 Mouse: NCG GemPharmatech (Nanjing, China) Strain #:T001475 Mouse:CD45.1 Jackson Laboratories Strain #: 002014 RRID:IMSR_JAX:002014 Mouse:OT-II Jackson Laboratories Strain #:004194 RRID:IMSR_JAX:004194 (Continued on next page) e2 iScience 28, 112800, July 18, 2025 .. The Irf4 f/f mouse strain was bought from the Jackson Laboratory (Bar Harbor, ME).

    Magnetic Beads:

    Article Title: H3K27-specific demethylase orchestrates the phenotypic and functional plasticity of intestinal ILC3s.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Alexa Fluor TM 594 Donkey Anti-Rabbit IgG (H + L) Cell Signaling Cat# A21207; RRID: AB_2340621 Normal Rabbit IgG (2729) Cell Signaling Cat# 2729S; Anti-Rabbit IgG (H + L) Sigma-Aldrich Cat# SAB3700883; Bacterial and virus strains DH5α Chemically Competent Cell Tsingke Biotech Cat#TSC-C14 Citrobacter rodentium strain DBS100 ATCC Stock# 51459 Candida albicans strain SC5314. .. Guanghua Huang Lab, Fudan University Gifted from Prof. Guanghua Huang Chemicals, peptides, and recombinant proteins Fixable Viability Dye 450 eBioscience Cat#65-0863-14 Fixable Viability Stain 510 BD Cat#564406 FBS Gibco Cat#10270 PMA Sigma-Aldric Cat# P1585 DTT, Molecular Grade (Dry Powder) Promega Cat#V3151 Ionomycin Sigma-Aldric Cat# I3909 Polybrene Millpore Cat# TR-1003-G DNase I Sigma Cat# DN25 Collagenase VIII Sigma Aldrich Cat#C2139 Percoll Cytiva Cat#17-0891-01 PrimeSTAR MAX Premix TAKARA Cat# R045A Mouse IL-23 Recombinant Protein Peprotech Cat# 14-8231-63 IL-1β Peprotech Cat# SRP8033 Interleukin-7 Peprotech Cat# I4892 IL-6 Peprotech Cat# SRP3330 Murine SCF Peprotech Cat#250-03-50 RetroNectin Takara Cat#T100A/B Critical commercial assays BD Rhapsody TM WTA Amplification Kit BD Biosciences Cat# 633801 AMPure® XP magnetic beads Beckman Cat# A63880 Qubit TM dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q32851 BD Rhapsody TM Cartridge Reagent Kit BD Biosciences Cat# 633731 BD Rhapsody TM cDNA Kit BD Biosciences Cat# 633773 Deposited data scRNA-seq This paper GSE271288 CUT&Tag sequencing This paper GSE281331 Experimental models: cell lines HEK 293T ATCC Cat#ACS-4500 Plat E Shanghai Institutes for Biological Sciences Gifted from Prof. Xiaolong Liu Experimental models: organisms/strains Mouse:Kdm6a flox/flox (Utx) Jackson Laboratories Strain #:021926 RRID:IMSR_JAX:021926 Mouse:Kdm6b flox/flox (Jmjd3) Jackson Laboratories Strain #:029615 RRID:IMSR_JAX:029615 Mouse:Rorc-cre Jackson Laboratories Strain #: 022791 RRID:IMSR_JAX:022791 Mouse: NCG GemPharmatech (Nanjing, China) Strain #:T001475 Mouse:CD45.1 Jackson Laboratories Strain #: 002014 RRID:IMSR_JAX:002014 Deposited data RNAseq data This paper CRA033355 CUT&Tag This paper CRA033268 (Continued on next page) Cell Reports 45, 116862, January 27, 2026 17 .. The Jmjd3 f/f and Utx f/f mouse strain were purchased from the Jackson Laboratory (Bar Harbor, ME).

    Article Title: The transcription factor IRF4 regulates the homeostasis and function of intestinal ILC3s.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER IRF-4 Rabbit mAb (D9P5H) Cell Signaling Cat# 15106T; RRID: AB_2798709 Anti-BATF (EPR21911) Abcam Cat# ab221146; RRID: AB_3665801 Alexa Fluor TM 594 Donkey Anti-Rabbit IgG (H + L) Cell Signaling Cat# A21207; RRID: AB_2340621 Normal Rabbit IgG (2729) Cell Signaling Cat# 2729S; RRID:AB_1031062 Anti-Rabbit IgG (H + L) Sigma-Aldrich Cat# SAB3700883; RRID: AB_3697514 Bacterial and virus strains DH5α Chemically Competent Cell Tsingke Biotech Cat#TSC-C14 Citrobacter rodentium strain DBS100 ATCC Stock# 51459 Candida albicans strain SC5314. .. Guanghua Huang Lab, Fudan University Gifted from Prof. Guanghua Huang Chemicals, peptides, and recombinant proteins Fixable Viability Dye 450 eBioscience Cat#65-0863-14 Fixable Viability Stain 510 BD Cat#564406 FBS Gibco Cat#10270 PMA Sigma-Aldric Cat# P1585 DTT, Molecular Grade (Dry Powder) Promega Cat#V3151 Ionomycin Sigma-Aldric Cat# I3909 Polybrene Millpore Cat# TR-1003-G DNase I Sigma Cat# DN25 Collagenase VIII Sigma Aldrich Cat#C2139 Percoll Cytiva Cat#17-0891-01 PrimeSTAR MAX Premix TAKARA Cat# R045A Mouse IL-23 Recombinant Protein Peprotech Cat# 14-8231-63 IL-1β Peprotech Cat# SRP8033 Interleukin-7 Peprotech Cat# I4892 IL-6 Peprotech Cat# SRP3330 Murine SCF Peprotech Cat#250-03-50 RetroNectin Takara Cat#T100A/B Critical commercial assays BD Rhapsody TM WTA Amplification Kit BD Biosciences Cat# 633801 AMPure® XP magnetic beads Beckman Cat# A63880 Qubit TM dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q32851 BD Rhapsody TM Cartridge Reagent Kit BD Biosciences Cat# 633731 BD Rhapsody TM cDNA Kit BD Biosciences Cat# 633773 Deposited data scRNA-seq This paper GSE271288 CUT&Tag sequencing This paper GSE281331 Experimental models: Cell lines HEK 293T ATCC Cat#ACS-4500 Plat E Shanghai Institutes for Biological Sciences Gifted from Prof. Xiaolong Liu Experimental models: Organisms/strains Mouse:Irf4 flox/flox Jackson Laboratories Strain #:009380 RRID:IMSR_JAX:009380 Mouse:Rorc-cre Jackson Laboratories Strain #: 022791 RRID:IMSR_JAX:022791 Mouse: NCG GemPharmatech (Nanjing, China) Strain #:T001475 Mouse:CD45.1 Jackson Laboratories Strain #: 002014 RRID:IMSR_JAX:002014 Mouse:OT-II Jackson Laboratories Strain #:004194 RRID:IMSR_JAX:004194 (Continued on next page) e2 iScience 28, 112800, July 18, 2025 .. The Irf4 f/f mouse strain was bought from the Jackson Laboratory (Bar Harbor, ME).

    Sequencing:

    Article Title: H3K27-specific demethylase orchestrates the phenotypic and functional plasticity of intestinal ILC3s.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Alexa Fluor TM 594 Donkey Anti-Rabbit IgG (H + L) Cell Signaling Cat# A21207; RRID: AB_2340621 Normal Rabbit IgG (2729) Cell Signaling Cat# 2729S; Anti-Rabbit IgG (H + L) Sigma-Aldrich Cat# SAB3700883; Bacterial and virus strains DH5α Chemically Competent Cell Tsingke Biotech Cat#TSC-C14 Citrobacter rodentium strain DBS100 ATCC Stock# 51459 Candida albicans strain SC5314. .. Guanghua Huang Lab, Fudan University Gifted from Prof. Guanghua Huang Chemicals, peptides, and recombinant proteins Fixable Viability Dye 450 eBioscience Cat#65-0863-14 Fixable Viability Stain 510 BD Cat#564406 FBS Gibco Cat#10270 PMA Sigma-Aldric Cat# P1585 DTT, Molecular Grade (Dry Powder) Promega Cat#V3151 Ionomycin Sigma-Aldric Cat# I3909 Polybrene Millpore Cat# TR-1003-G DNase I Sigma Cat# DN25 Collagenase VIII Sigma Aldrich Cat#C2139 Percoll Cytiva Cat#17-0891-01 PrimeSTAR MAX Premix TAKARA Cat# R045A Mouse IL-23 Recombinant Protein Peprotech Cat# 14-8231-63 IL-1β Peprotech Cat# SRP8033 Interleukin-7 Peprotech Cat# I4892 IL-6 Peprotech Cat# SRP3330 Murine SCF Peprotech Cat#250-03-50 RetroNectin Takara Cat#T100A/B Critical commercial assays BD Rhapsody TM WTA Amplification Kit BD Biosciences Cat# 633801 AMPure® XP magnetic beads Beckman Cat# A63880 Qubit TM dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q32851 BD Rhapsody TM Cartridge Reagent Kit BD Biosciences Cat# 633731 BD Rhapsody TM cDNA Kit BD Biosciences Cat# 633773 Deposited data scRNA-seq This paper GSE271288 CUT&Tag sequencing This paper GSE281331 Experimental models: cell lines HEK 293T ATCC Cat#ACS-4500 Plat E Shanghai Institutes for Biological Sciences Gifted from Prof. Xiaolong Liu Experimental models: organisms/strains Mouse:Kdm6a flox/flox (Utx) Jackson Laboratories Strain #:021926 RRID:IMSR_JAX:021926 Mouse:Kdm6b flox/flox (Jmjd3) Jackson Laboratories Strain #:029615 RRID:IMSR_JAX:029615 Mouse:Rorc-cre Jackson Laboratories Strain #: 022791 RRID:IMSR_JAX:022791 Mouse: NCG GemPharmatech (Nanjing, China) Strain #:T001475 Mouse:CD45.1 Jackson Laboratories Strain #: 002014 RRID:IMSR_JAX:002014 Deposited data RNAseq data This paper CRA033355 CUT&Tag This paper CRA033268 (Continued on next page) Cell Reports 45, 116862, January 27, 2026 17 .. The Jmjd3 f/f and Utx f/f mouse strain were purchased from the Jackson Laboratory (Bar Harbor, ME).

    Article Title: The transcription factor IRF4 regulates the homeostasis and function of intestinal ILC3s.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER IRF-4 Rabbit mAb (D9P5H) Cell Signaling Cat# 15106T; RRID: AB_2798709 Anti-BATF (EPR21911) Abcam Cat# ab221146; RRID: AB_3665801 Alexa Fluor TM 594 Donkey Anti-Rabbit IgG (H + L) Cell Signaling Cat# A21207; RRID: AB_2340621 Normal Rabbit IgG (2729) Cell Signaling Cat# 2729S; RRID:AB_1031062 Anti-Rabbit IgG (H + L) Sigma-Aldrich Cat# SAB3700883; RRID: AB_3697514 Bacterial and virus strains DH5α Chemically Competent Cell Tsingke Biotech Cat#TSC-C14 Citrobacter rodentium strain DBS100 ATCC Stock# 51459 Candida albicans strain SC5314. .. Guanghua Huang Lab, Fudan University Gifted from Prof. Guanghua Huang Chemicals, peptides, and recombinant proteins Fixable Viability Dye 450 eBioscience Cat#65-0863-14 Fixable Viability Stain 510 BD Cat#564406 FBS Gibco Cat#10270 PMA Sigma-Aldric Cat# P1585 DTT, Molecular Grade (Dry Powder) Promega Cat#V3151 Ionomycin Sigma-Aldric Cat# I3909 Polybrene Millpore Cat# TR-1003-G DNase I Sigma Cat# DN25 Collagenase VIII Sigma Aldrich Cat#C2139 Percoll Cytiva Cat#17-0891-01 PrimeSTAR MAX Premix TAKARA Cat# R045A Mouse IL-23 Recombinant Protein Peprotech Cat# 14-8231-63 IL-1β Peprotech Cat# SRP8033 Interleukin-7 Peprotech Cat# I4892 IL-6 Peprotech Cat# SRP3330 Murine SCF Peprotech Cat#250-03-50 RetroNectin Takara Cat#T100A/B Critical commercial assays BD Rhapsody TM WTA Amplification Kit BD Biosciences Cat# 633801 AMPure® XP magnetic beads Beckman Cat# A63880 Qubit TM dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q32851 BD Rhapsody TM Cartridge Reagent Kit BD Biosciences Cat# 633731 BD Rhapsody TM cDNA Kit BD Biosciences Cat# 633773 Deposited data scRNA-seq This paper GSE271288 CUT&Tag sequencing This paper GSE281331 Experimental models: Cell lines HEK 293T ATCC Cat#ACS-4500 Plat E Shanghai Institutes for Biological Sciences Gifted from Prof. Xiaolong Liu Experimental models: Organisms/strains Mouse:Irf4 flox/flox Jackson Laboratories Strain #:009380 RRID:IMSR_JAX:009380 Mouse:Rorc-cre Jackson Laboratories Strain #: 022791 RRID:IMSR_JAX:022791 Mouse: NCG GemPharmatech (Nanjing, China) Strain #:T001475 Mouse:CD45.1 Jackson Laboratories Strain #: 002014 RRID:IMSR_JAX:002014 Mouse:OT-II Jackson Laboratories Strain #:004194 RRID:IMSR_JAX:004194 (Continued on next page) e2 iScience 28, 112800, July 18, 2025 .. The Irf4 f/f mouse strain was bought from the Jackson Laboratory (Bar Harbor, ME).

    RNA sequencing:

    Article Title: H3K27-specific demethylase orchestrates the phenotypic and functional plasticity of intestinal ILC3s.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Alexa Fluor TM 594 Donkey Anti-Rabbit IgG (H + L) Cell Signaling Cat# A21207; RRID: AB_2340621 Normal Rabbit IgG (2729) Cell Signaling Cat# 2729S; Anti-Rabbit IgG (H + L) Sigma-Aldrich Cat# SAB3700883; Bacterial and virus strains DH5α Chemically Competent Cell Tsingke Biotech Cat#TSC-C14 Citrobacter rodentium strain DBS100 ATCC Stock# 51459 Candida albicans strain SC5314. .. Guanghua Huang Lab, Fudan University Gifted from Prof. Guanghua Huang Chemicals, peptides, and recombinant proteins Fixable Viability Dye 450 eBioscience Cat#65-0863-14 Fixable Viability Stain 510 BD Cat#564406 FBS Gibco Cat#10270 PMA Sigma-Aldric Cat# P1585 DTT, Molecular Grade (Dry Powder) Promega Cat#V3151 Ionomycin Sigma-Aldric Cat# I3909 Polybrene Millpore Cat# TR-1003-G DNase I Sigma Cat# DN25 Collagenase VIII Sigma Aldrich Cat#C2139 Percoll Cytiva Cat#17-0891-01 PrimeSTAR MAX Premix TAKARA Cat# R045A Mouse IL-23 Recombinant Protein Peprotech Cat# 14-8231-63 IL-1β Peprotech Cat# SRP8033 Interleukin-7 Peprotech Cat# I4892 IL-6 Peprotech Cat# SRP3330 Murine SCF Peprotech Cat#250-03-50 RetroNectin Takara Cat#T100A/B Critical commercial assays BD Rhapsody TM WTA Amplification Kit BD Biosciences Cat# 633801 AMPure® XP magnetic beads Beckman Cat# A63880 Qubit TM dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q32851 BD Rhapsody TM Cartridge Reagent Kit BD Biosciences Cat# 633731 BD Rhapsody TM cDNA Kit BD Biosciences Cat# 633773 Deposited data scRNA-seq This paper GSE271288 CUT&Tag sequencing This paper GSE281331 Experimental models: cell lines HEK 293T ATCC Cat#ACS-4500 Plat E Shanghai Institutes for Biological Sciences Gifted from Prof. Xiaolong Liu Experimental models: organisms/strains Mouse:Kdm6a flox/flox (Utx) Jackson Laboratories Strain #:021926 RRID:IMSR_JAX:021926 Mouse:Kdm6b flox/flox (Jmjd3) Jackson Laboratories Strain #:029615 RRID:IMSR_JAX:029615 Mouse:Rorc-cre Jackson Laboratories Strain #: 022791 RRID:IMSR_JAX:022791 Mouse: NCG GemPharmatech (Nanjing, China) Strain #:T001475 Mouse:CD45.1 Jackson Laboratories Strain #: 002014 RRID:IMSR_JAX:002014 Deposited data RNAseq data This paper CRA033355 CUT&Tag This paper CRA033268 (Continued on next page) Cell Reports 45, 116862, January 27, 2026 17 .. The Jmjd3 f/f and Utx f/f mouse strain were purchased from the Jackson Laboratory (Bar Harbor, ME).



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